{"id":985,"date":"2026-08-02T07:27:22","date_gmt":"2026-08-02T11:27:22","guid":{"rendered":"https:\/\/cellpathway.com\/?page_id=985"},"modified":"2026-08-07T15:45:19","modified_gmt":"2026-08-07T19:45:19","slug":"facilities","status":"publish","type":"page","link":"https:\/\/cellpathway.com\/?page_id=985","title":{"rendered":"Facilities"},"content":{"rendered":"<section class=\"cp-facilities-page\" aria-label=\"Laboratory facilities and resources\">\n<nav class=\"cp-facilities-map\" aria-label=\"Facilities sections\">\n    <a href=\"#facilities-overview\"><span class=\"cp-facilities-map-icon\" aria-hidden=\"true\"><svg viewBox=\"0 0 48 48\" focusable=\"false\"><circle cx=\"24\" cy=\"24\" r=\"17\"\/><circle cx=\"24\" cy=\"24\" r=\"4\"\/><path d=\"M24 7v6m0 22v6M7 24h6m22 0h6M15 15l4 4m10 10l4 4m0-18l-4 4M19 29l-4 4\"\/><\/svg><\/span><span class=\"cp-facilities-map-label\">Overview<\/span><\/a><br \/>\n    <a href=\"#lab-equipment\"><span class=\"cp-facilities-map-icon\" aria-hidden=\"true\"><svg viewBox=\"0 0 48 48\" focusable=\"false\"><path d=\"M18 5h12M21 5v12L10 36a5 5 0 0 0 4 7h20a5 5 0 0 0 4-7L27 17V5\"\/><path d=\"M15 33h18M18 28h12\"\/><circle cx=\"34\" cy=\"14\" r=\"5\"\/><path d=\"M34 6v3m0 10v3m8-8h-3m-10 0h-3m13-6l-2 3m-6 6l-2 3m10 0l-2-3m-6-6l-2-3\"\/><\/svg><\/span><span class=\"cp-facilities-map-label\">Lab Equipment<\/span><\/a><br \/>\n    <a href=\"#lab-tour\"><span class=\"cp-facilities-map-icon\" aria-hidden=\"true\"><svg viewBox=\"0 0 48 48\" focusable=\"false\"><rect x=\"5\" y=\"8\" width=\"38\" height=\"28\" rx=\"4\"\/><path d=\"M20 17l11 7-11 7zM16 43h16M24 36v7\"\/><\/svg><\/span><span class=\"cp-facilities-map-label\">Lab Tour<\/span><\/a><br \/>\n    <a href=\"#institutional-environment\"><span class=\"cp-facilities-map-icon\" aria-hidden=\"true\"><svg viewBox=\"0 0 48 48\" focusable=\"false\"><path d=\"M5 18h38L24 6zM8 40h32M6 44h36M11 18v22m8-22v22m10-22v22m8-22v22\"\/><\/svg><\/span><span class=\"cp-facilities-map-label\">Institutional Environment<\/span><\/a><br \/>\n    <a href=\"#lcms\"><span class=\"cp-facilities-map-icon\" aria-hidden=\"true\"><svg viewBox=\"0 0 48 48\" focusable=\"false\"><path d=\"M5 39h38M8 36V24m6 12V11m6 25V29m6 7V7m6 29V21m6 15V16\"\/><path d=\"M9 9h9M13 5v8M35 7c0 4-4 7-4 11a4 4 0 0 0 8 0c0-4-4-7-4-11z\"\/><\/svg><\/span><span class=\"cp-facilities-map-label\">LCMS<\/span><\/a><br \/>\n  <\/nav>\n<section class=\"cp-facilities-subsection cp-facilities-overview\" aria-labelledby=\"facilities-overview\">\n<h2 id=\"facilities-overview\" class=\"cp-facilities-section-title\" tabindex=\"-1\">Overview<\/h2>\n<p class=\"cp-facilities-lead\">The Salomon Laboratory combines dedicated wet-lab and tissue-culture space, substantial computational infrastructure, direct access to the adjacent Brown Proteomics Core Facility, and university resources in imaging, genomics, flow cytometry, structural biology, and biophysics. Together, these capabilities support mechanistic studies of T cell and CAR T cell signaling from sample preparation through quantitative analysis.<\/p>\n<figure class=\"cp-facilities-video cp-lmm-location-video\">\n      <video controls loop muted playsinline autoplay preload=\"metadata\" data-accessible-autoplay aria-describedby=\"cp-lmm-location-video-caption\"><source src=\"https:\/\/cellpathway.com\/wp-content\/uploads\/2026\/08\/70-ship-lmm-salomon-lab-silent.mp4\" type=\"video\/mp4\">Your browser does not support embedded video. <a href=\"https:\/\/cellpathway.com\/wp-content\/uploads\/2026\/08\/70-ship-lmm-salomon-lab-silent.mp4\">Download the silent location video<\/a>.<\/video><figcaption id=\"cp-lmm-location-video-caption\">Salomon lab at the Laboratories of Molecular Medicine at Brown University.<\/figcaption><\/figure>\n<\/section>\n<section class=\"cp-facilities-subsection\" aria-labelledby=\"lab-equipment\">\n<h2 id=\"lab-equipment\" class=\"cp-facilities-section-title\" tabindex=\"-1\">Lab Equipment<\/h2>\n<div class=\"cp-facilities-grid\">\n<section class=\"cp-facility-card\">\n      <span class=\"cp-facility-icon\" aria-hidden=\"true\"><svg viewBox=\"0 0 48 48\" focusable=\"false\"><path d=\"M7 42h34M11 42V16h26v26M17 16V7h14v9M17 24h5m4 0h5m-14 7h5m4 0h5M21 42v-6h6v6\"\/><\/svg><\/span><\/p>\n<h3>Wet-lab capabilities<\/h3>\n<ul>\n<li>Dedicated wet-lab and tissue-culture space with laminar-flow hoods and CO2 incubators<\/li>\n<li>PCR, electrophoresis, microscopy, refrigerated centrifugation, and monitored cold storage<\/li>\n<li>SpeedVac, lyophilizer, offline HPLC, and specialized preparation tools for quantitative proteomics<\/li>\n<\/ul>\n<\/section>\n<section class=\"cp-facility-card\">\n      <span class=\"cp-facility-icon cp-flux-capacitor-icon\" aria-hidden=\"true\"><svg viewBox=\"0 0 48 48\" focusable=\"false\"><rect x=\"6\" y=\"5\" width=\"36\" height=\"38\" rx=\"5\"\/><circle cx=\"24\" cy=\"25\" r=\"4\"\/><path d=\"M24 21V11M21 14h6M20 22l-8-8M12 18v-6h6M28 22l8-8M36 18v-6h-6M24 29v8M20 37h8\"\/><path d=\"M10 39h28\"\/><\/svg><\/span><\/p>\n<h3>Advanced equipment<\/h3>\n<ul>\n<li>Orbitrap Ascend Tribrid Mass Spectrometer with FAIMS<\/li>\n<li>Vanquish Neo UHPLC with EasySpray and NanoFlex sources<\/li>\n<li>TECAN A200 automated sample preparation<\/li>\n<li>Q Exactive Orbitrap with UltiMate3000 nanoRSLC for pilot studies<\/li>\n<\/ul>\n<\/section>\n<section class=\"cp-facility-card\">\n      <span class=\"cp-facility-icon\" aria-hidden=\"true\"><svg viewBox=\"0 0 48 48\" focusable=\"false\"><rect x=\"8\" y=\"6\" width=\"32\" height=\"10\" rx=\"2\"\/><rect x=\"8\" y=\"19\" width=\"32\" height=\"10\" rx=\"2\"\/><rect x=\"8\" y=\"32\" width=\"32\" height=\"10\" rx=\"2\"\/><circle cx=\"14\" cy=\"11\" r=\"1.5\"\/><circle cx=\"14\" cy=\"24\" r=\"1.5\"\/><circle cx=\"14\" cy=\"37\" r=\"1.5\"\/><path d=\"M20 11h14M20 24h14M20 37h14\"\/><\/svg><\/span><\/p>\n<h3>Computational infrastructure<\/h3>\n<ul>\n<li>Dedicated proteomics servers in a climate-controlled room with UPS and emergency power<\/li>\n<li>MASCOT, MaxQuant, Proteome Discoverer, SpectroMine, Spectronaut, DIA-NN, and FragPipe analysis resources<\/li>\n<li>Automated parallel HTAPP workflows, 52 TB redundant storage, and nightly institutional backup<\/li>\n<\/ul>\n<\/section><\/div>\n<\/section>\n<section class=\"cp-facilities-subsection\" aria-labelledby=\"lab-tour\">\n<h2 id=\"lab-tour\" class=\"cp-facilities-section-title\" tabindex=\"-1\">Lab Tour<\/h2>\n<figure class=\"cp-facilities-video cp-lab-tour\">\n      <video controls loop muted playsinline autoplay preload=\"metadata\" data-accessible-autoplay aria-describedby=\"cp-lab-tour-caption\"><source src=\"https:\/\/cellpathway.com\/wp-content\/uploads\/2026\/08\/salomon-lab-tour-cinematic-bright-fitted-silent.mp4\" type=\"video\/mp4\">Your browser does not support embedded video. <a href=\"https:\/\/cellpathway.com\/wp-content\/uploads\/2026\/08\/salomon-lab-tour-cinematic-bright-fitted-silent.mp4\">Download the silent lab tour video<\/a>.<\/video><figcaption id=\"cp-lab-tour-caption\">A tour of the Salomon Laboratory\u2019s wet-lab space and the adjacent Brown Proteomics Core Facility. The closing portion shows the dedicated servers directly connected to the mass spectrometers for fully automated proteomic data analysis using software developed in the laboratory.<\/figcaption><\/figure>\n<\/section>\n<section class=\"cp-facilities-subsection cp-institutional-environment\" aria-labelledby=\"institutional-environment\">\n<h2 id=\"institutional-environment\" class=\"cp-facilities-section-title\" tabindex=\"-1\">Institutional Environment<\/h2>\n<p>The laboratory is embedded in Brown University\u2019s Laboratories for Molecular Medicine, where wet-lab research is supported by nearby shared facilities, specialized instrumentation, and experienced scientific staff. These resources extend our experimental reach beyond proteomics while keeping complementary approaches close to the laboratory.<\/p>\n<div class=\"cp-shared-core-grid\" aria-label=\"Selected Brown University shared research facilities\">\n<section class=\"cp-shared-core-card\">\n<h3>Leduc Bioimaging Facility<\/h3>\n<p>Confocal, multiphoton, high-content fluorescence, electron microscopy, and specialized imaging sample preparation.<\/p>\n<\/section>\n<section class=\"cp-shared-core-card\">\n<h3>Genomics Core at LMM<\/h3>\n<p>Sequencing, quantitative PCR, plate scanning, and LI-COR infrared imaging for nucleic acid and protein measurements.<\/p>\n<\/section>\n<section class=\"cp-shared-core-card\">\n<h3>Flow Cytometry and Sorting<\/h3>\n<p>Multiparameter flow analysis, high-throughput acquisition, and cell sorting with FACSAria, FACSCelesta, and Cytek Aurora systems.<\/p>\n<\/section>\n<section class=\"cp-shared-core-card\">\n<h3>Structural Biology and Biophysics<\/h3>\n<p>500, 600, and 850 MHz NMR spectroscopy, isothermal titration calorimetry, microscale thermophoresis, circular dichroism, and fluorescence measurements.<\/p>\n<\/section><\/div>\n<\/section>\n<section class=\"cp-facilities-subsection\" aria-labelledby=\"lcms\">\n<h2 id=\"lcms\" class=\"cp-facilities-section-title cp-facilities-video-heading\" tabindex=\"-1\">LCMS<\/h2>\n<figure class=\"cp-facilities-video\">\n      <video controls loop muted playsinline autoplay preload=\"metadata\" data-accessible-autoplay aria-describedby=\"cp-facilities-video-caption\"><source src=\"https:\/\/cellpathway.com\/wp-content\/uploads\/2026\/07\/orbitrap-ascend-dasilva.mp4\" type=\"video\/mp4\">Your browser does not support embedded video. <a href=\"https:\/\/cellpathway.com\/wp-content\/uploads\/2026\/07\/orbitrap-ascend-dasilva.mp4\">Download the Orbitrap Ascend video<\/a>.<\/video><figcaption id=\"cp-facilities-video-caption\"><strong>Advanced mass spectrometry at Brown.<\/strong> Dr. Nicholas DaSilva with the Thermo Scientific Orbitrap Ascend Tribrid Mass Spectrometer. The instrument is located in the shared instrument room immediately adjacent to the Salomon Laboratory, giving Salomon Lab trainees and personnel direct, 24\/7 walk-up access. The Orbitrap Ascend was acquired with NIH support (1S10OD036295-01; PI: A. Salomon).<\/figcaption><\/figure>\n<\/section>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>Overview Lab Equipment Lab Tour Institutional Environment LCMS Overview The Salomon Laboratory combines dedicated wet-lab and tissue-culture space, substantial computational infrastructure, direct access to the adjacent Brown Proteomics Core Facility, and university resources in imaging, genomics, flow cytometry, structural biology, and biophysics. Together, these capabilities support mechanistic studies of T cell and CAR T cell [&hellip;]<\/p>\n","protected":false},"author":0,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-985","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/cellpathway.com\/index.php?rest_route=\/wp\/v2\/pages\/985","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cellpathway.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/cellpathway.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"replies":[{"embeddable":true,"href":"https:\/\/cellpathway.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=985"}],"version-history":[{"count":20,"href":"https:\/\/cellpathway.com\/index.php?rest_route=\/wp\/v2\/pages\/985\/revisions"}],"predecessor-version":[{"id":1075,"href":"https:\/\/cellpathway.com\/index.php?rest_route=\/wp\/v2\/pages\/985\/revisions\/1075"}],"wp:attachment":[{"href":"https:\/\/cellpathway.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=985"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}